E26防治植物根癌病的效果及其稳定性初步研究

Preliminary Study on Effectiveness and the Stability of E26 on Controlling Crown Gall Disease

选取不致病的葡萄土壤杆菌 (Agrobacterium vitis) E2 6菌株 ,以 HL B- 2和 K84菌株做对照 ,对不同来源的 36株根癌菌进行室内及温室条件下的抑菌及防治效果试验。结果表明 :室内抑菌检测中 ,E2 6的抑菌谱要广于 HL B- 2和 K84 ,对 36株根癌菌中的 30株有拮抗作用 ,抑菌率为 83.3%。在温室防治检测中 ,生防菌与根癌菌 1∶ 1混合接种指示植物向日葵时 ,E2 6对 85.7%的供试菌株有防治效果 ;与对照生防菌相比 ,尤以防治葡萄根癌病最为有效。单菌落传代研究表明 :E2 6后代菌株与初始菌株在产素及生长速度方面均无显著差异 ,表明 E2

Nonpathogenic strain E26 isolated from the crown gall of grapevines was used in the study for the control effectiveness on crown gall diseases. Plating and greenhouse tests revealed that E26 could control 83.3% and 85.7% of the tested strains respectively, which suggested that E26 was an effective strain to control crown gall, especially for grapevine crown galls. Agrocin assay by transferring strain E26 on plates indicated that agrocin production of E26 is a stable property.