吲哚乙酸处理桉树插条后氧化酶活性及同工酶变化与生根关系的比较研究

Study on the Relationship between the Changes of Activities and Isoenzymes of Oxidases and the Rooting of Eucalyptus Cuttings Treated with Indoleacetic Acid

尾叶桉 ML A无性系 (简称 MLA)为难生根植物 ,尾叶桉 U6无性系 (简称 U6)和刚果 12号桉W5无性系 (简称 W5)为易生根植物。 MLA的插条内的 POD、IAAO活性较 U6、W5的高 ,而 PPO活性比 U6、W5的低。用 IAA处理桉树的插条后 ,在扦插生根的不同阶段 ,插条内的 POD、PPO、IAAO活性呈现规律性的变化。蛋白质含量呈上升趋势。POD、PPO同工酶谱带也随生根的进程出现增多现象。本文讨论了 3种氧化酶与桉树插条生根的关系。

Eucalyptus urophylla MLA clone (MLA) was difficult to root species. E. urophylla U 6 clone (U 6) and E. ABL 12 W 5 clone (W 5) were relatively easy to root species. The activities of peroxidase (POD) and indoleacetic acid oxidase (IAAO) in MLA were higher than that in U 6 and W 5. But the activity of polyphenol oxidase (PPO) in MLA was lower than that in U 6 and W 5. After the cuttings of Eucalyptus were treated with indoleacetic acid (IAA), the activities of POD, PPO and IAAO and the content of protein increased regularly in different stages of rooting. The isoenzymes of POD and PPO increased after treatment with IAA. The relationship between the oxidases and rooting of Eucalyptus cuttings was discussed.