红花对小鼠骨髓和人淋巴细胞微核形成的影响

Effect of Flos Carthami on the Micronucleus of the Mouse Bone marrow and the Human Peripheral Lymphocyte

本文采用小鼠骨髓和人淋巴细胞微核试验方法测试了新疆产的红花的诱变性。结果表明,红花水提取物和乙醚提取物均能诱发小鼠骨髓嗜多染红细胞(PCE)微核率升高。红花水提取物的剂量在4～20ml/kg体重范围内,乙醚提取物剂量在1～4ml/kg体重诱发的小鼠骨髓PCE微核率分别为5.16±0.83‰～8.33±0.76‰,3.83±0.48～5.17±0.83‰,与阴性对照(2.5±0.34‰)比较,差异极显著(P<0.01)。红花水提取物亦能诱发人淋巴细胞微核率升高,在实验范围内(0.5～1.5μl/ml)诱发的微核率(3‰～6‰)与阴性对照(2‰)比较有显著性差异(P<0.05)。

Micronucleus test on the mouse bone marrow polychromatic erythrocytes(PCEs) and the human peripheral lymphocytes was used in this paper to detect the mutagenicity of Flos Carthami which was yielded in Xinjiang. The results showed that the rates of micronucleus on the mouse bone marrow PCEs were enchanced by the water and ether extracts of Flos Cartharni. The rates of micronucleus on the mouse bone marrow PCEs were 5.16±0.83‰～8.33±0.76‰ and 3.83±0.48‰～5.17±0.83‰ when the doses of water extracts and ether extracts of Flos Carthami were at 4～20g/kg and 20～80g/kg respectively. The rate of micronucleus on the mouse bone marrow PCEs in the negative control group was 2.5±0.34‰ The difference between the negative control and experimental, groups was significant(P<0.01). The rares of micronucleus on the hunan peripheral lymphoeytes were also enchanced by the water extracts of Flos Carthami. The rates of micronucleus were 3～6‰when the doses were 0.5～1.5μl/ml. Compared with the rates of negative control group(2‰)the difference was significant.