四君子汤复方总多糖口服和注射给药对免疫功能影响的对比研究

Effects of Oral Administration and Intraperitoneal Injection of Total Polysaccharide of Sijunzi Decoction on Mouse Immune Function

目的 :研究四君子汤复方总多糖经口服和注射给药的免疫活性的差异。方法 :采用环磷酰胺造成免疫抑制模型 ,观察四君子汤复方总多糖对小鼠免疫器官重量、溶血素生成反应和迟发型超敏反应的影响 ,同时观察对正常淋巴细胞增殖的影响。结果 :四君子汤复方总多糖能够明显对抗由环磷酰胺诱导的免疫抑制 ,使小鼠免疫器官重量减轻 ,溶血素生成反应减弱和迟发型超敏反应降低明显改善 ,促进淋巴细胞增殖 ,且口服效果有优于注射的趋势。结论 :四君子汤复方总多糖对小鼠体液和细胞免疫功能具有促进作用 ,同时也提示多糖口服给药引起免疫效应有其独特性。文中还对口服多糖引起全身免疫反应的可能机理进行了探讨。

Objective: To study difference of the immune response between oral administration and intraperitoneal injection of total polysaccharide of Sijunzi decoction (SJTP) in mice. Methods: The immunosuppressed mice model was established by intraperitoneal injection of cyclophosphamide (CY). The effects of SJTP on the weight of immune organs,hemolysin response and delayed type hypersensitivity (DTH) were observed,and the influences on normal mouse spleen lymphocytic proliferation of mice were also studied. Results:SJTP can remarkably counteract the immunosuppression induced by CY, decrease the weight loss immune organs,increase the hemolysin response and DTH,and promote the proliferation of lymphocytes.The effect of oral administration is superior to intrapertoneal injection.Conclusion:SJTP can improve the humoral and cellalar immunity of mice.The immune activities resulting from oral administration of SJTP have some special characteristics,and its possible mechanism is also discussed.