ALF大鼠eNOSmRNA、iNOSmRNA表达的实验研究

EXPERIMENTAL STUDY OF eNOSmRNA、 iNOSmRNA EXPRESSION IN ACUTE LIVER FAILURE RAT

目的 :从分子水平探讨急性肝功能衰竭 (acuteliverfailure,ALF)大鼠机体重要脏器eNOSmRNA、iNOSm RNA表达的变化 ,揭示其在ALF时多脏器功能障碍中的作用。方法 :通过切除大鼠 90 %肝脏制备急性肝功能衰竭模型 ,并在ALF 6h及应用NO供体或 /和NOS抑制剂 6h分别处死动物 ,取其肝、肺、肾、小肠和大肠制备组织切片 ,应用原位杂交方法测定每一组织eNOSmRNA和iNOSmRNA活性表达情况。结果 :eNOSmRNA表达在ALF 6h肺、大肠显示增加 ,而iNOSmRNA在肝、肺、肾、小肠和大肠表达明显增加 ;ALF 12h、应用NO供体和 /或NOS抑制剂 ,肺、大肠表达eNOSmRNA下降 ,同时肝、肺、肾、小肠和大肠iNOSmRNA表达均明显降低。结论 :急性肝功能衰竭早期 (6h)肺、大肠eNOSmRNA和肝、肺、肾、小肠和大肠iNOSmRNA表达显著增高 ,其翻译合成iNOS诱导产生的大量NO ,可能参与早期脏器功能的损害 ;而应用NO供体和 /或NOS抑制剂 ,eNOSmRNA和iNOSmRNA的表达均明显降低 。

Objective:To reveal the expression of eNOSmRNA、iNOSmRNA in Acute liver Failure (ALF) Rat's important tissues at molecule level and observe the effects on the Rat's multiple organs dysfunction (MODS).Methods:Establish the ALF model by resecting 90% of rat liver and using NO precursor or/and NOS inhibitor to induce or inhibit NO production in vivo.At the time of 6h of ALF,and 6h of using NO precursor or/and NOS inhibitor,the animals are sacrificed respectively to make liver,lung,kidney,small intestine and large intestine into tissue sections,the expression of eNOSmRNA and iNOSmRNA in various tissues is determined with in situ hybridization.Results:The expression of eNOSmRNA in lung and large intestine at 6h of ALF increased signiificantly,the expression of iNOSmRNA in liver,lung,kidney,small and large intestines incresed significatnly too;the expression of eNOSmRNA was lower in lung and large intestine at 12h and when using NO precursor or/and NO inhibitor,while the expression of iNOSmRNA in lung,liver,kidney,small and large intestines was also lower simultaneously.Conclusions:The significantly higher expression of iNOSmRNA and eNOSmRNA have important effect on the damage of important organs at early stage of ALF,while the lower expression of iNOSmRNA and eNOSmRNA can alleviate the damage of important organs when using NO precursor or/and NO inhibitor.