雷公藤对异位角膜移植抗排斥反应的研究

Experimental study on corneal allograft rejection with tripterygium wolfordii hook

目的：采用雷公藤对角膜移植排斥反应进行实验性治疗。 方法以 Ｗｉｓｔａｒ大鼠异种角膜异位移植为模型，将 ３６只鼠随机分为治疗组及对照组，并在术后ｄ２、ｄ５、ｄ７、ｄ１０、ｄ１４分别取出植片做病理切片，检测外周血Ｔ淋巴细胞亚群及Ｔ淋巴细胞集落形成数。 结果雷公藤可推迟排斥反应的发生时间（排斥时间 ７．５±１．０７ｄ，对照组 ５．４±０． ９７ｄ）；有明显减少外周血中辅助 Ｔ淋巴细胞百分率的作用（术后 ｄ５治疗组 ＣＤ４＋／ＣＤ８＋为 １． ４２±０． ２７，对照组为１， ９６±０． ２６）；对大鼠外周血 Ｔ淋巴细胞集落形成能力无明显影响。 结论雷公藤多甙是一种有效的免疫抑制剂，能明显抑制角膜移植排斥反应发生时间及 ＣＤ４＋／ＣＤ８＋比值的上升。

Objective: The tripterygium wolfordii hook was used in comeal allograft rejection experiment. Methods The wistar rats were used as model of hetero graft-heterotopic graft rejection. thirty-six rats were randomly divided into the treatment and the control groups. On the 2nd. 5th. 7th and the 14th day after the transplantation,the transplanted corneas were removed for pathological examinations. The peripheral blood was col- feeted for the analysis of T cell subgroups and T lymphocyte colony forming unit(T-CFU) assay. Results Tripterygium wolfordii hook could delay the development of comeal graft rejection and significantly reduce the Percentage of T-helper cells. On the other hand,it could not weaken the gathering capacity of the peripheral T-cells.. Conclusion Tripterygium wolfordii hook is an effective immnuosuppressant.