青岛地区丙型肝炎病毒基因型分布情况调查

Investigation on Hepatitis C Virus Genotype Distribution in Qingdao Areas

为了解青岛地区丙型肝炎病毒(HCV)基因型分布情况.采用逆转录一套式聚合酶链反应和限制性片段长度的多态性分析方法,对154份HCV-RNA阳性血清进行了HCV-5’末端非编码区酶切分型.结果:HCVⅡ型占82.5%、HCVⅢ型占16.9%、Ⅱ/Ⅲ混合型占0.6%;在各临床类型中,HCV型的感染率随着肝病的进展而逐渐增加.提示:HCVⅡ型为青岛地区HCV感染的优势株,并且与肝病的进展密切相关.今后在丙肝的诊断、治疗及疫苗开发等研究方面,应将HCVⅡ型作为重点.

To reflect hepatitis C virus genotype distribution in Qingdao areas, 154 cases of HCV-RNA positive sera were classified according to HCV-5'NC cleavage site by RT-PCRand RFLP. The results showed that of 154 HCV-RNA positive sera type Ⅱ -HCV was 82. 5% and type Ⅲ -HCV 16. 9% .type Ⅱ / Ⅲ -HCV 0. 6%. Infection chance of type I -HCV rises with liver disease development in all clinical types. This finding indicated that type Ⅱ -HCV was obviously a dominant strain of HCV in Qingdao areas and be connected with liver diseases development closely. So type Ⅱ -HCV infection will be focal point in diagnosis therapy of hepatitis C and exploitation of vaccine.