肾康注射液与苯那普利对肾小球系膜细胞外基质抑制作用的血清学对比研究

Inhibiting Contrast Study of Shenkang Injection and Benazepril on Human Glomerular Mesangial Cell Extracellular Matrix

目的 :探讨肾康注射液与苯那普利对人肾小球系膜细胞外基质 (ECM )不同成分的抑制作用强度。方法 :采用体外培养的人肾小球系膜细胞 (MC)技术和血清药理学方法 ,观察具有降逆泄浊、益气活血作用的中药肾康注射液及苯那普利对ECM不同成分的影响。结果 :肾康注射液与苯那普利对ECM中纤维连接蛋白 (FN)、层粘连蛋白(LN)和Ⅳ型胶原 (ColⅣ )均具有一定的抑制作用 ,但在等效剂量浓度下 ,肾康注射液对MC的抑制作用优于苯那普利。结论 :MC是肾康注射液发挥治疗作用的重要靶细胞之一 ,抑制ECM聚积可能是该方延缓肾小球硬化的重要作用机制之一。

To explore the intensity of inhibitory function of Shenkang injection (SKI), a Chinese herbal recipe, and benazepril on the proliferation of human glomerular mesangial cell (MC). Methods: The effect of SKI and benazepril on the proliferation of human MC was observed by using the cultured MC in vitro and serum pharmacological assay. Results: Both SKI and benazepril could inhibit the proliferation of human MC, but in equal dosage consistency, SKI showed a better inhibitory effect than that of benazepril. Conclusion: MC is the important target cell for the action of SKI, the inhibiting on proliferation might be one of the essential mechanisms of SKI in retarding glomerulosclerosis.