革兰阴性杆菌诱导型β-内酰胺酶试验及药敏检测结果

The measurement of induction β lactamases in Gram negative rods and results analysis in the susceptibility tests

对１３５株革兰阴性杆菌进行诱导型β－内酰胺酶检测的同时对产酶组与非产酶组药敏结果加以分析。结果，产诱导型β－内酰胺酶细菌６０株，占４４％，其中以铜绿假单胞菌、产气肠杆菌、弗劳地枸橼酸杆菌、阴沟肠杆菌检出率最高，依次为５６％、５０％、４４％和４０％。产酶组与非产酶组对三代头孢菌素耐药率比较，发现二者无显著差异，提示常规药敏试验未能完全反映细菌耐药情况，诱导型β－内酰胺酶的检测，可以补充常规药敏不足。同时本文通过ＷＨＯＮＥＴ－３统计软件对产诱导酶的菌进行配对散点图分析，发现亚胺培南与氨基糖甙类和喹喏酮类抗菌药物有较强的互补性，提示在临床调整用药时，可以选择抗菌药物不同的配对联合用药。

This paper are reported the measurement of induction β lactamases in 135 strains Gram negative rods and assays their susecptible results between induction β lactamases group and noninduction β lactamases group. 60 of 135 strains are induction β lactamases(44%), of those, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii and Enterboacter cloacae are 56%, 50%, 44%, 40%, respectively. There are no significantly difference between induction β lactamases group and the third generation cephalosporins and prompts the routine suscepitibility tests are not enough to reflect clinical resistant situations, but the measurement of induction β lactamases can supplement this deficiency. On the other hand, this file assays the induction β lactamases group by scatter with WHONET 3 statistics software. Results shows there are strongly complementarity among imipenem, aminoglycosides and fluoroqinolones.