人肝癌组织中MCT1,MCT2基因mRNA表达的半定量研究

Semi-quantitative analysis of MCT1 and MCT2 genes mRNA expression in human hepatocellular carcinoma

目的 探讨单羧酸转运蛋白 1(MCT1)、单羧酸转运蛋白 2 (MCT2 )mRNA在人肝癌及癌旁肝组织中的表达及意义。方法 应用RT PCR及电泳条带光密度比较法对 2 5例肝癌病人的肝癌及癌旁肝组织中MCT1,MCT2mRNA的表达进行半定量分析。结果 肝癌及癌旁肝组织的MCT1mRNA的表达显著高于MCT2。肝癌组织中MCT1,MCT2mRNA的表达显著高于癌旁肝组织。结论 MCT1。

Objective To study the mRNA expression of MCT1 and MCT2 genes in human hepatocellular carcinoma (HCC) and paracarcinoma liver tissue (PCLT). Methods The semi-quantitative analysis of MCT1 and MCT2 genes mRNA expression in human HCC and PCLT was conducted by RT-PCR method and electrophoresis band opacity density (OD) comparison analysis method in 25 patients with HCC. Results The mRNA expression of MCT1 was significantly higher than MCT2 in HCC and PCLT, in HCC the mRNA expression of MCT1 and MCT2 genes were significant higher than that in PCLT. Conclusions The high expression of mRNA of MCT1 and MCT2 genes in HCC indicates that these genes may take a significant role on lactate and other monocarboxylate transmembrane transportation and on pH regulation in tumor cells.