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γ-干扰素对肝癌细胞株Hep-G2 Fas,Bcl-2表达的影响 被引量：2

Effect of γ-interferon on the expresson of Fas, Bcl-2 on Hep-G2 hepatocellular carcinoma cells
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摘要 目的　观察γ 干扰素 (γ IFN)对肝癌细胞株Hep G2细胞 (Hep G2 )表达Fas ,Bcl 2的影响。方法　应用γ IFN预处理Hep G2 ,用阿霉素诱导凋亡 ,MTT法分析细胞死亡率 ,电镜观察凋亡 ,免疫组织化学法研究γ IFN作用于Hepp G2细胞过程中Fas ,Bcl 2的表达情况。结果　γ IFN预处理的Hep G2表达Fas增强 ,表达Bcl 2减弱 (P <0 .0 5 ) ;γ IFN预处理后Hep G2细胞对阿霉素的敏感性提高 (P <0 .0 5 )。结论　γ IFN对肝癌细胞株Hep G2Fas ,Bcl 2表达有影响 ,并可提高肝癌细胞对阿霉素的敏感性。 Objective To investigate the influence of γ-interferon (γ-IFN) on liver cancer cell line (Hep-G2). Methods Observing the expression of Fas and Bcl-2 by γ-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results (1) γ-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P<0.05), and the sensitivity of pretreated hep-G2 cell to adrimysin was increased. Conclusions γ-IFN can rise the sensitivity of Hep-G2 to adrimysin via regulating the expression of Fas and Bcl-2.
作者 郑岩松 林永堃 吕新生 石铮
机构地区 福建医科大学附属第一医院普外科 中南大学湘雅医院普外科
出处 《中国普通外科杂志》 CAS CSCD 2001年第2期139-141,共3页 China Journal of General Surgery
基金 福建省政府科研基金资助! (99J 0 0 6)
关键词 Γ-干扰素 药理学 基因表达 FAS基因 BCL-2基因 细胞凋亡 肿瘤细胞 肝癌细胞株 INTERFERON/pharmaco GENES EXPRESSION/drug eff GENE,FAS GENE,Bcl-2 TUMOR CELLS,CULTURED/drug eff CELL LINE,TRANSFORMED/drug eff APOPTOSIS CARCINOMA,HEPATOCELLCILAR
分类号 R735.7 [医药卫生—肿瘤]
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参考文献7

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同被引文献14

  • 1高德宗,孙靖中,李永刚,邵立华,李进涛.Bcl-2和Caspase-3调控他莫昔芬诱导的ER阴性乳腺癌细胞凋亡[J].中国普通外科杂志,2005,14(9):683-686. 被引量：4
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中国普通外科杂志
2001年 第2期

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