实验性糖尿病骨质疏松的扫描电镜观察

Scanning electron microscopic observation on experimental osteoporosis in diabetes mellitus rats

目的 :研究糖尿病性骨质疏松的骨结构改变。方法 :用四氧嘧啶注入Wister大鼠尾静脉的方法 ,制成糖尿病动物模型。分别取成模后 4和 6个月的大鼠胫骨 ,在扫描电镜下观察骨结构的改变。结果 :糖尿病大鼠的胫骨的骨密质变薄 ,表面有峰窝状的骨质吸收。骨小梁数量明显减少 ,骨质松脆、断裂 ,骨髓腔扩大。结论 :糖尿病可引起骨的三维结构破坏和骨质疏松 ,随之造成骨的强度下降及骨折。

Objective:To investigate the bone architectural changes on osteoporosis in diabete mellitus rats induced by alloxan.Methods:Wister rats were injected with alloxan(mesoxalyl urea) into coccygeal veins to set up animal models with diabetes mellitus.After four and eight months respectively,tibiae of the rat were observed with scanning electron microscope.Results:Cortical bones of tibia in rats with diabetes became thin,there was bone resorption area like honeycomb on the surface.Trabecular bone of the tibia were manifested with fewness,fragility and disconnection.The cavities of bone marrow enlarged.Conclusion:Diabetes mellitus can cause destruction of bone three dimensional structure and osteoporosis,followed with a decline in the mechanical strength and fracture.