增生性瘢痕和瘢痕疙瘩中PTK活性测定

Activity of protein tyrosine kinase in hypertrophic scar and keloid tissue

目的 测定增生性瘢痕和瘢痕疙瘩组织中PTK活性变化 ,探讨PTK在瘢痕形成中的作用。方法 利用活化的PTK催化底物多肽磷酸化 ,再计数底物中掺入32 P的放射活性的原理 ,测定增生性瘢痕、瘢痕疙瘩、成熟瘢痕和正常皮肤组织中PTK活性。结果 增生性瘢痕和瘢痕疙瘩组织中PTK的活性显著高于正常成熟瘢痕和正常皮肤 (P <0 .0 0 1,分别高出正常皮肤 15 0 .80 %和 3 13 .80 % ) ,瘢痕疙瘩高于增生性瘢痕 (P <0 .0 0 1,高出 65 .0 1% )而成熟瘢痕和正常皮肤间没有差异 (P >0 .0 5 )。结论 瘢痕组织中PTK活性升高且与增生程度相关 ,PTK介导生长刺激的信号转导使细胞增殖和合成物质的功能增强而发生瘢痕增生。

Objective To monitor the activity of protein tyrosine kinase (PTK) in hypertrophic scar (HS) and keloid (K) tissue and to study effects of PTK on scar formation. Methods The principle of activated PTK to transfer phosphorus ( 32 p) into substrate was used to calculate activity of PTK by detecting radioactivity of substrate with scintillation counter. Six cases of HS and K were measured. Mature scar (MS) and normal skin (NS) were used as control.Results Activity of PTK in HS and K was obviously elevated to compare with ones in MS and NS ( P <0.001); one of K was more higher. When proliferation of scar tissue deceased activity of PTK reduced consistently. The difference between MS and NS is also significant ( P <0.001).Conclusion Activity of PTK in scar tissue was elevated and increasing degree of PTK might be related to proliferate level of scar.