检测人动脉瘤组织中肺炎衣原体特异性抗原的临床意义

Clinical Significance of Detecting Chlamydia Pneumoniae-Specific Antigen in Human Artery Atheroma Tissues

目的 :探讨肺炎衣原体 (Cpn)特异性抗原与动脉瘤的关系。方法 :利用直接免疫荧光 (IF)方法检测新鲜 -冰冻或福尔马林 -固定标本中Cpn特异性抗原的存在。结果 :新鲜 -冰冻标本中Cpn特异性抗原的阳性率是 :腹主动脉瘤组为 83.0 % (15 /18) ,正常血管组 12 .5 % (1/ 8) ,P <0 .0 1。此外 ,用IF方法检测了 40例档案保存的石蜡包埋冠状动脉瘤标本和正常血管组织切片 ,Cpn特异性抗原检出率分别是 :87.5 % (35 / 40 )和 0 (P <0 .0 0 1)。结论 :Cpn特异性抗原的高检出率见于人动脉瘤体组织中 ,推测Cpn引起的动脉粥样硬化可能是形成人动脉瘤的病因之一。

Objective: To investigate the relationship between Cpn-specific antigen and artery atheroma. Methods: Fresh-frozen or formalin-fixed specimens were tested for the presence of Cpn-specific antigen using direct immunofluorescence (IF). Results: The positive rate of Cpn-specific antigen was 83.0%(15/18)in eighteen fresh-frozen abdominal artery atheroma specimens, and the positive rate for Cpn antigen was 12.5%(1/8)in eight fresh-frozen normal artery tissues, P <0.01. A total 40 archival paraffin-embedded coronary artery atheroma specimens were examined by IF, the positive rate for Cpn-specific antigen was 87.5%(35/40), and 10 formalin-fixed normal artery tissue sections were negative, ( P <0.001). Conclusion: A high prevalence of C. pneumoniae-specific antigen is found in artery atheroma, which suggest that atherosclerosis related to Cpn may be a possible causation of artery atheroma.