锌诱导扁藻类金属硫蛋白的初步研究

Preliminary study on the induction of zinc to playmomas subcordiformis metallothionein-like

用不同浓度的ＺｎＳ４对绿藻门（ｃｈｌｏｒｏｐｈｔａ）、绿藻纲（ｃｈｌｏｒｏｐｈｙｃｅａｅ）、塔形藻亚目（Ｐｙｒａｍｉｍｏｎａ－ｄｌｎｅａｅ）、局藻科（Ｐｌａｔｙｍｏｎａｄａｃｅａｅ）、扁藻属（Ｐｌａｔｙｍｏｎａｓ）、亚心形扁藻（Ｐｌａｔｙｍｏｎａｓ ｓｕｂｃｏｒｄｉｆｃｒｍｉｓ）进行诱导培养，当 ＺｎＳＯ４浓度在１ｍｍｏｌ·Ｌ～－１以上时，随着ＺｎＳＯ４浓度的升高，扁藻的生长率逐渐降低，扁藻不能耐受８ｍｍｏｌ·Ｌ－１以上的ＺｎＳＯ４。对类ＭＴ提取液进行原子吸收测定，结果表明扁藻类ＭＴ的合成随着ＺｎＳＯ４浓度的升高而增大，诱导效果明显。用１ｍｍｏｌ·Ｌ～－１ＺｎＳＯ４诱导培养扁藻，每克湿藻能获得 ２．１ｍｇ类ＭＴ。

When Playmonas cardiformis is exposed to different ZnSO4 concentration, its growth rate decreases gradually with the increase of ZnSO4 concentration which is more than 1 mmol·L^-1. More than 8 mmol·L^-1 ZnSO4 can lead to the death of playmonas subcordi- formis. But the production of MT-like increases with the increase of ZnSO4 concentration by AAS determination. The effect of indution is remarkable. The production of MT-like reaches 2. 1ing per gram wet weight when the concentration of ZnSO4 is 1 mmol·L^-1.