麦芽醇治疗大鼠不完全性脑缺血再灌注损伤的实验研究

Study of Therapeatic Effects of Maltol on Focal Cerebral Ischemia-Reperfusion Damages in Rats

目的研究麦芽醇对大鼠海马组织不完全性脑缺血再灌注损伤的保护作用。方法Wistar大鼠双侧颈总动脉夹闭 30min ,造成不完全脑缺血 ,松开动脉夹即为再灌注。大鼠随机分为三组 :①假手术对照组 (SOC) ,相同手术操作 ,但不夹闭动脉 ;②缺血再灌注组 (IR) ,再灌注 1h ;③麦芽醇治疗组 (MT) ,夹闭动脉前后静脉注射 70 0 μmol/L麦芽醇生理盐水。 3组动物均于再灌注后测海马组织中的丙二醛(MDA)值、超氧化物歧化酶 (SOD)和ATP酶活性。结果缺血再灌注组MDA明显升高 (P <0 .0 1 ) ,SOD、ATP酶活性明显降低 (P <0 .0 1 )。MT能降低升高的MDA值 ,改善ATP酶活性 (P <0 .0 1 ) ,但不能改善SOD活性。结论麦芽酶能清除氧自由基 ,减轻脂质过氧化反应 ,改善ATP酶活性 ,从而减轻海马组织缺血再灌注损伤。

Objective To investigate the protective effects of maltol (MT)on focal cerebral ischemia-reperfusion damages in hippocampal structure in rats.Methods An ischemia model of wistar rats was established by clipping bilateralcervical arteries for 30 minutes,and then reperfusion was made through removing the clippings.Rats were randomly divided into three groups:①Sham operated control group(SOC),which is surgically exposed bilateralcervical arteries only.②Ischemia-reperfusion group(IR).③MT treated group,700μmol/L maltol(MT) normal saline was administered through intravenous injection before clipping and reperfusion.The malondialdehyde (MDA) content,superoxide dismutase (SOD) activity and ATPase activity were detected 1 hour after reperfusion for all 3 groups.Results MDA content rose obviously (P<0.01) andSOD,ATPase activity reduced obviously(P<0.01) in IR group.MT can diminish MDA content(P<0.01) and inprove ATPase activity (P<0.01),but can't improre SOD activity.Conclusion MT can clear oxygenic radical,reduce the reaction of lipid-peroxidation and improre ATPase activity. So MT can decrease damage in the IR injury of hippocampal structure.