大白鼠提睾肌血管中CGRP免疫反应神经纤维的分布

Distribution of CGRP-immunoreactive fibers in the cremaster arteries of rats

目的 研究大鼠提睾肌血管壁降钙素基因相关肽 (Calcitoningene relatedpeptide ,CGRP)免疫反应性纤维的分布特点。方法 采用免疫组织化学ABC法对大白鼠提睾肌动脉各级分支的CGRP免疫反应阳性纤维的分布特点进行观察和研究。结果 大白鼠提睾肌动脉各级分支血管均有CGRP广泛分布 ,一、二级动脉壁上呈密集颗粒状分布 ,局部有密集的团块。三级以下除颗粒状可见到横行或网格状分布 ,且CGRP阳性反应纤维的密度随分支级别的增加而减少。在毛细血管壁上仍可见到网格状阳性纤维的分布。结论 CGRP阳性纤维的分布可能与大鼠提睾肌动脉的生理调节和微循环有关。

Objective To study distribution of the calcitonin gene related peptide immunoreactive fibers in the cremaster arteries of the rat. Methods The distribution of the calcitonin gene related peptide immunoreactive fibers in the cremaster arteries had been studied by means of ABC technique.Results The calcitonin gene related peptide immunoreactive fibers were observed on all branches of cremaster arteries.The immunoreative fibers in first and second branches of the arteries exhibited the denser grains and denser mass,but in third branches,the network of the fibers was obvious.The density of the immunoreactive fibers reduced following the bifurcations of the arteries.The network of the fibers also could be found in the capillary.Conclusion The results demonstrated that the distribution of calcitonin gene related peptide immunoreactive fibers might be related to the physiological regulation and microcirculation of the cremaster arteries.It formed the morphological basis for experimental model of researching nerve regulation in the microcirculation.