脑梗塞患者血浆同型半胱氨酸水平与叶酸、B_(12)的关系

Plasma homocysteine to folate and vitamin B_(12) in cerebral infarction

目的 研究同型半胱氨酸 (HCY)与脑梗塞的关系及叶酸、维生素B1 2 (B1 2 )对HCY水平的影响。方法 采用高效液相色谱荧光检测法测定 40例脑梗塞患者和 2 0例同龄健康对照组血浆HCY浓度 ,用SimulTRAC SNB放射法测定两组血清叶酸、B1 2 的含量。结果 ①脑梗塞患者血浆HCY浓度 (2 4 0 2 μmol/L± 1 1 2 4 μmol/L)高于对照组 (1 4 0 μmol/L± 4 48μmol/L) (P <0 0 1 ) ;②脑梗塞患者叶酸水平 (4 82 μg/L± 1 97μg/L)显著低于对照组 (6 88μg/L± 2 85μg/L) (P <0 0 0 1 ) ;脑梗塞患者B1 2 含量 (2 4 7 56ng/L± 1 58 72ng/L)略低于对照组 (2 70 31ng/L±1 98 39ng/L) ,但无显著差异 (P >0 0 5) ,Pearson相关分析显示两组HCY浓度与叶酸水平均呈负相关性 ,与B1 2 水平无显著相关。结论 脑梗塞患者血浆HCY浓度高于同龄健康人 ;HCY浓度与叶酸水平呈负相关性 ,与B1 2

Objective To investigate the plasma homocysteine level and cerebral infarction and the association between plasma homocysteine level and levels of serum folate and vitamin B 12 . Methods Levels of plasma homocysteine of 40 cerebral infarction patients and 20 healthy subjects were determined using high performance liquid chromatography with fluorescence detection. Serum folate and vitamin B 12 concentrations were measured by SimulTRAC SNB radioassay.Results ①The mean plasma level of homocystine was higher in patients than that in control[(24 02±11 24)?μmol/L versus(14 10±4 48)?μmol/L, P <0 01];②The level of serum folate was significantly lower in patients than that in control[(4 82±1 97)?μg/L versus(6 88±2 85)?μg/L, P <0 001].The level of serum B 12 was slightly lower in patients[(247 56±158 72)?ng/L] than that in control[(270 31±198 39)?ng/L],but the difference was not statistically significant ( P >0 05).Person correlation anslysis revealed that plasma homocysteine level showed a strong inverse association with folate level in patients( r =-0 39, P =0 01)and in controls( r =-0 46, P =0 03).Vitamin B 12 did not show correlation to plasma homocysteine in cases or controls( P >0 05).Conclusion ①Plasma homocysteine level in cerebral infarction patients is higher than that in the same age health.②There is a strong inverse association between plasma homocysteine and folate.The association between plasma homocysteine and vitamin B 12 is not statistically significant.