普乐林诱导HL-60细胞分化和凋亡研究

Study on the differentiation and apoptosis of HL-60 cell line induced by Puerarin

目的 :研究中药葛根的提取物普乐林对HL 6 0细胞的诱导分化和促凋亡作用。方法 :采用硝基苯唑氮兰(NBT)还原、NBT/MTT值及细胞表面抗原CD11b表达 ,观察细胞分化程度 ;通过细胞形态、DNA片段电泳、流式细胞(FCM)DNA含量分析检测细胞凋亡。结果 :80 μg .ml 1,16 0 μg .ml 1和 32 0 μg·ml-1普乐林对HL 6 0细胞具有诱导分化效应 ;且 32 0 μg .ml 1普乐林与 1μmol.L 1全反式维甲酸效果相当。 32 0 μg .ml 1和 6 40 μg.ml 1普乐林分别作用HL 6 0细胞 36h ,可出现典型的细胞凋亡形态。DNA凝胶电泳出现片段化的梯状带谱 ;普乐林同时可影响HL 6 0细胞的细胞周期进展 ,且随其浓度的增高 ,亚G1期细胞所占比例增加。结论 :普乐林具有诱导HL 6 0细胞分化及凋亡的双重作用。

Objective To investigate the differentiation of HL 60 cells induced by different doses of Purerarin(PR) comparing with all trans retinoic acid(ATRA)and to see if PR can induce apoptosis of HL 60 cells. Methods Cell differentiation was analyzed by NBT reduction, the ratio of NBT/MTT and CD11b ,apoptosis by morphology, DNA electrophoresis, and flow cytometry(FCM). Results 80 μg.ml 1 , 160 μg.ml 1 , 320?μg.ml 1 PR could induce differentiation of HL 60 cells, no significant difference was observed between the cells treated with 1?μmol.L 1 ATRA and 320?μg.ml 1 PR. Treated with 320?μg.ml 1 , 640?μg.ml 1 PR, HL 60 cells exhibited a morphological characteristic of apoptosis and typical DNA ladder on gel electrophoresis. FCM analysis showed that PR could interfere with cell cycle in HL 60 cells, with a increased ratio of sub G1 in HL 60 cells. Conclusion PR exerts effect on differentiation and induces apoptosis in HL 60 cells.