骨肉瘤患者外周血单个核细胞IL-6和NO的测定

Study on in vitro nitric oxide and interleukin-6 levels Induced from peripheral Mononuclear cells in patients with osteosarcoma

从 32例骨肉瘤患者和 2 0例健康志愿者的外周血中分离出单个核细胞 ,经脂多糖诱生培养 48h ,收集上清液 ,用酶联免疫吸附法测定IL 6的含量 ,用镀铜镉还原法测定NO的含量。结果发现 :骨肉瘤组IL 6和NO水平明显高于对照组 (P <0 .0 1)。骨肉瘤患者IL 6与NO水平呈正相关 (r =0 .6 5 2 ,P <0 .0 1)。提示骨肉瘤患者外周血单个核细胞处于激活状态可产生高水平的IL 6及NO ;可能与骨肉瘤的发病机制有关。

Peripheral blood mononuclear cells from 32 cases of osteosarcoma and 20 normal controls were separated and induced by lipopolysaccharide, followed by 48 hour incubation in vitro, then the supernatant were collected. The levels of IL 6 were measured by enzyme linked immunosorbent assays, the concentrations of NO were measured by Griess methods. The results were as follows: The concentrations of IL 6 and NO were significantly higher than those in normal contrls (P<0.01). There were positive correlation between the levels of IL 6 and NO in patients with osteosarcoma (r=0.652, P<0.01). The results suggest that the immune function of peripheral mononuclear cell in patients with osteosarcoma was disordered. It may activate peripheral mononuclear cells to produce high levels of IL 6 and NO, which may take part in the pathogenesis of osteosarcoma.