血清β-羟丁酸测定方法的建立及其在糖尿病酮症酸中毒中的意义

Establishment of method for determining serum beta-hydroxybutyric acid and its meaning in diabetes ketosis acidism

目的 :建立酶速率法测定血清 β 羟丁酸 ,并探讨 β 羟丁酸在糖尿病酮症酸中毒中的意义。方法 :采用酶速率法在日立 7170A全自动生化分析仪上测定 6 0例正常人、30例糖尿病人和 8例酮症患者血清中 β 羟丁酸含量。结果 :本方法测定 β 羟丁酸回收率 10 8% ,线性范围 0～ 4.0mmol.L 1,批内CV2 .2 % ,批间CV2 .6 % ;糖尿病酮症酸中毒患者血清β 羟丁酸明显升高。结论 :采用酶速率法测定血清 β 羟丁酸快速、准确、线性范围宽 ,可作为糖尿病酮症酸中毒特异和快速的诊断试验。

Objective This study was to establish enzyme rate method for determining serum beta hydroxybutyric acid ,and to discuss its meaning in diabetes ketosis acidism. Methods Enzyme rate method was used to determine serum beta hydroxybutyric acid in 60 cases of normals, 30 cases of diabetes ,and 8 cases of ketosis acidism by Hitachi 7170A autochemistry analyzer. Results The recovery rate is 108%,the linerity extent 0 4.0 mmol.L 1 , the coefficient of variation within run is 2.2%and day to day is 2.6%. Conclusion The enzyme rate method for determining serum beta hydroxybutyric acid save time, gives accurate results and has wide linerity,so it can used for diagnosis of diabetes ketosis acidism.