原癌基因表达与哮喘气道炎症细胞浸润的关系

The relationship between Proto-oncogenes expression and airway inflammatory cell infiltration in asthma

目的 :观察原癌基因表达与气道炎症细胞浸润的关系。方法 :卵蛋白致敏豚鼠 ,复制哮喘模型。Dot-blot、Northern -blot分子杂交及免疫组织化学技术观察哮喘发作前、后豚鼠气道上皮及肺组织中c -fos、c -myc、c-jun和c-sis的表达及其与炎症细胞浸润的关系。结果 :正常豚鼠气道及肺组织中c -fos、c -mycmRNA无或很少表达 ,极少炎症细胞浸润。哮喘发作后 ,豚鼠气道上皮及肺组织c -fos、c -mycmRNA表达明显增加。免疫组织化学研究显示Fos、Myc、Jun及Sis在正常豚鼠气道及肺组织低水平表达 ,哮喘发作后即刻 ,4种原癌基因表达产物明显增加 ,气道内有少量淋巴细胞、嗜酸性粒细胞及中性粒细胞浸润 ;12 - 2 4h ,上述细胞浸润加重 ,粘膜下层及粘膜上皮内以中性粒细胞和嗜酸性粒细胞浸润为主。结论 :原癌基因表达与气道炎症在哮喘发病中有密切关系。

AIM: To investigate the relationship between inflammatory cell infiltration and proto-oncogenes expression in asthma. METHODS: Guinea pigs were used as asthma models challenged by ovoglobulin. Dot-blot, Northern-blot and immunochemical techniques were used to detect the expression of c-fos, c-myc, c-jun and c-sis. Inflammatory cell infiltration was showed by pathologic study.RESULTS: c-fos and c-myc mRNA could not be detected or expressed at very low level in control group. Those were greatly increased after the animals are challenged by ovoglobulin. Immunochemical study showed that Fos, Myc, Jun and Sis expressed at low level in control group, and those were increased after the challenge. There was little inflammatory cell infiltration in control group. Lymphocyte, neutrophil and eosinophil were detected immediately after the challenge, a great number of inflammation cells could be seen after 12-24 h of the challenge. Majority of neutrophil and eosinophil were under mucosa or in epithelium in airway. CONCLUSION: Oncogenes expression had strong relationship with airway inflammation.