摘要
目的 :了解白血病病人异基因外周血干细胞移植后淋巴细胞TCRVβ基因片段选择性扩增的情况。方法 :采用RT -PCR扩增 5例CML和 2例AML移植后患者外周血的单个核细胞的TCRVβ 2 4个基因片段 ,分析其TCRVβ基因的表达情况。 结果 :不同标本中仅检测到 2 - 8个VβT细胞基因片段 ,不同患者中缺乏的TCRVβ基因片段不尽相同。结论 :移植后病人外周血淋巴细胞TCRVβ基因片段部分受抑制 。
AIM: To investigate restricted expansion of TCR Vβ gene repertoire in patients with leukemia following allogeneic hematopoietic stem cell transplantation. METHODS: TCR Vβ subfamily genes in peripheral blood mononuclear cells from 7 cases of leukemia was amplified using RT-PCR. RESULTS: Only two-eight fragments of Vβ genes were detected in samples from these patients, and the detected fragments are different in different patients. CONCLUSION: TCR complexes were abnormal in all patients, part of the genes were seletively expansed and part of them were suppressed after transplantation.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2001年第4期305-307,共3页
Chinese Journal of Pathophysiology
基金
广东省科委重点攻关项目! (No .199817)
广东省卫生厅医学科研基金!资助 (No.A19980 17)