血小板生成素Ⅱ体内生物活性研究

Study on biological activity of thrombopoietinⅡ in vivo

目的 :探讨血小板生成素受体的两种新配体—血小板生成素Ⅱ (TPOⅡ )在体内的生物学活性。方法 :以Babl c纯系小鼠为实验对象 ,通过腹腔连续 7d分组注射提纯的TPOⅡ配体Ⅰ、人工合成的TPOⅡ配体Ⅱ及重组的人血小板生成素 ,并于第 7d末测定小鼠外周血小板数量 ,分析TPOⅡ体内的生物学活性。结果 :在实验第 7d ,TPOⅡ配体Ⅰ实验组小鼠外周血小板数明显高于同期阴性对照组 (P <0 0 5 ) ,与rhTPO阳性对照组相比则无明显差异 (P >0 0 5 ) ;第 14d ,TPOⅡ的两个实验组的血小板数均较同期阴性对照组明显升高 (P <0 0 1) ;与阳性对照组相比亦无明显差异 (P >0 0 5 )。而且随实验时间的延长 ,TPOⅡ实验组及阳性对照组血小板数均呈升高趋势。结论 :提纯的TPOⅡ配体Ⅰ促进小鼠外周血小板的产生 ,其升高血小板的能力与rhTPO无明显差别。

AIM: To investigate the biological activity of thrombopoietin Ⅱ(TPOⅡ) in vivo , which consists of two new kinds of ligand binding with thrombopoietin receptor. METHODS: Purified ligandⅠof TPOⅡ, artificial compound ligandⅡ of TPOⅡand rhTPO were injected into purebred Babl/c mice respectively in 7 days by intraperitoneal injection once for a day. Then the biological activity of TPOⅡ was analyzed by measuring peripheral platelet counts by the end of the seventh day. RESULTS: On the seventh day, the platelet counts of mice treated by ligandⅠof TPOⅡ were higher than that in the negative control group( P< 0.05), while not significantly different from the platelet counts of mice treated by rhTPO( P> 0.05). On the fourteenth day, the platelet counts increased in two all experimental groups of TPOⅡcompared with negative control group( P< 0.01), while not significantly different from the platelet counts of mice treated by rhTPO too( P> 0.05). Moreover the platelet counts of mice in two experimental groups of TPOⅡ and the positive group showed increase with experimental days. CONCLUSION: The purified ligandⅠof TPOⅡ had obvious activity in increasing platelet production, which is not different from the effect of rhTPO.