去交感神经状态对肝部分切除后肝再生的影响

Effects of sympathetic denervation on liver regeneration after partial hepatic resection

目的 建立去交感神经状态动物模型并探讨去交感神经状态下对肝切除后肝再生的影响。方法 雄性Wistar大鼠共90只 ,用 6 OHDA制作去交感神经状态动物模型。其中 3 0只大鼠分为实验组和对照组各 15例。按Higgins和Anderson方法加以改良 ,作肝左叶和肝中叶切除 (约占全肝的 68% )。实验组加做去交感神经模型。术后第 7天全部动物经抽血处死 ,计算相对肝重(HMI)、肝再生率的指数 (RLR)和有丝分裂指数 (MI)。肝脏DNA合成率用3 H标记胸腺嘧啶核苷 (3 H TdR)掺入法测得。结果 注射6 OHDA后 3～ 14d ,NE含量明显降低。行肝切除后两组大鼠术后 7d均无死亡 ,实验组大鼠HMI、RLR、MI和3 H TdRDNA掺入量较对照组均明显下降 (P <0 .0 1)。结论 6 OHDA可明显起到化学性去交感神经的效果。交感神经的存在与否对肝切除后肝再生具有明显影响 。

Objective To establish the animal model of denervation of sympathetic nerve and to explore the effects of denervation of the sympathetic nerve on liver regeneration after partial resection. Methods The animal model of denervation of sympathetic nerve was made with 6-OHDA. A total of thirty male Wistar rats were divided equally into experimental and control group. The left and middle lobe of liver were resected with improved Higgins and Anderson's method. Meanwhile, denervation was made in the experimental group. All the rats were killed by haemospasia on the 7 th day after operation. HMI, RLR and MI were measured. The rates of DNA synthesis were detected by 3H-TdR method. Results The concentration of NE decreased extremely on day 3 to day 14 after administration of 6-ONDA. No death happened in all the rats 7 days after liver resection. HMI, RLR, MI and 3H-TdR incorporation significantly decreased in experimental group compared with that in control (P<0.01). Conclusion The chemical denervation of sympathetic nerve can be aroused by administration of 6-OHDA. Regeneration of the liver is inhibited by the denervation of sympathetic nerves.