蛋白激酶亚型在肾小球肾炎中的表达及意义

Expressions of protein kinase C subtypes in glomerulonephritis and their significance

目的 检测蛋白激酶C(ProteinkinaseC ,PKC)的α、β2、ε 3种亚型在肾小球肾炎中的表达 ,并探讨其在发病机制中的意义。方法 应用免疫组织化学技术检测 16例系膜增生性肾炎 (MsPGN)、12例非增生性肾炎 (nonPGN)病人肾穿刺标本及 6例正常肾组织中PKCα、2 β、ε的表达。 结果 MsPGN组肾小球部位PKCβ2表达明显高于nonPGN组 (P <0 .0 1)。 结论 PKCβ2与MsPGN的发病密切相关 。

Objective To investigate the expressions and significance of protein kinase C (PKC) subtype α, β2 and ε in the pathogenesis of glomerulonephritis. Methods Immunohistochemical assays was used to examine the expressions of PKC α, PKC β2 and PKC ε in biopsy specimens of kidney from patients with mesangial proliferative glomerulonephritis (MsPGN) (n=16),nonproliferative golmerulonephritis (nonPGN) (n=12),and in structurally normal kidneys (n=6). Results The PKC β2 expression in the glomerular was significantly higher in MsPGN patients than in nonPGN or normal kidney groups. Conclusion PKC β2 has a close association with MsPGN and might take part in the initiation of this renal disease.