微量免疫荧光试验和PCR在诊断肺炎衣原体急性感染中的应用

Application of microimmunofluorescence test and PCR in the diagnosis of acute Chlamydia pneumoniae infections

目的 探讨肺炎衣原体 (Cpn)急性感染在呼吸道疾病中的重要性。方法 用微量免疫荧光 (MIF)试验检测了 93例住院病人血清中Cpn抗体IgG ,同时用PCR测定了其中 5 5例病人咽试子标本中CpnDNA。结果 3 5 .5 %呼吸道疾病存在Cpn急性感染。在肺炎组抗体 (效价≥ 5 12 )阳性率为 47.6% ,提示 1998～ 1999年可能处在北京市Cpn流行期 ;在哮喘组的阳性率达到 5 0 % ;在肺心病组和肺癌组的阳性率分别为 5 0 .0 %和 2 6.3 %。PCR检测Cpn的阳性率仅为 9.1% ,MIF和PCR的检测结果存在差别。结论 检测Cpn抗体IgG有助于对Cpn急性感染进行诊断 。

Objective To explore the role of acute infection of Chlamydia pneumoniae (Cpn) in respiratory diseases. Methods Microimmunofluorescence test was used to detect IgG antibodies for Cpn in serum obtained from 93 inpatients and PCR was used to test Cpn in detection of Cpn DNA in throat specimens from 55 of the 99 patients. Results Acute Cpn infection was diagnosed in 35.5% of the respiratory diseases. Antibodies for Cpn (titer of ≥512) were present in 47.6% of the pneumonia group, which may suggest that during 1998 to 1999, Cpn caused an epidemic in Beijing. They were also present in 50% of asthma group, 50.0% of pulmonary heart disease group and 26.3% of lung cancer group. Only five patients (9.1%) were positive by PCR. There exists discrepancy between serological and PCR results. Conclusion Detection of IgG antibodies for Cpn conduces to diagnosis of acute Cpn infection and give advice for appropriate therapy.