母乳及婴儿食品对致病性大肠杆菌粘附的影响

Effects of breast milk and some infant foods on the adhesion of entero-pathogenic Escherichia coli (EPEC) to Hela cells and intestinal mucosa

目的 比较母乳、人工婴儿食品及其喂养的婴儿粪便对致病性大肠杆菌 (EPEC)粘附上皮细胞或肠粘膜的影响 ,以进一步阐明母乳喂养对婴儿肠道的保护作用。方法 通过细胞培养、细菌粘附实验、光镜和电镜等技术观察EPEC在含有母乳、牛奶、奶粉及母乳喂养和人工喂养的婴儿粪便滤过液的培养基中粘附HeLa细胞及肠粘膜的情况。结果 EPEC在含母乳的培养基中对HeLa细胞的粘附指数显著低于对照孔 ,而在含牛奶和奶粉的培养基中EPEC的粘附指数与对照孔比较无显著差别 ;母乳喂养婴儿粪便滤过液中的细菌粘附指数明显低于人工喂养婴儿粪便中的粘附指数。

Objective To investigate the effect of breast milk and some other kinds of infant foods on the adhesion of EPEC to Hela cells and intestinal mucosa so as to understand the possible protective mechanism of breast-feeding in the intestine of infants. Methods The adhesion of EPEC to Hela cells and intestinal mucosa were examined by cell culture, bacterial adherence test, light and electron microscopy in the mediums that contain breast milk, cow's milk and formula milk or contain ecal filtrate of the infants fed by breast milk and formula milk respectively. Results Adhesive index for EPEC to Hela cells was significantly lower in the medium containing breast milk than in control and so did in the fecal medium containing breast fed infants than artificial feeding infants. Conclusion Breast milk may inhibit the adhesion of EPEC to infant intestine.