交变电场下离子交换色谱分离过程

INVESTIGATIONS ON ION EXCHANGE CHROMATOGRAPHY CONDUCTED IN ALTERNATING ELECTRIC FIELD

以牛血清白蛋白在阴离子交换剂DEAE -SepharoseFastFlow上的吸附和解吸过程为例 ,考察了交变电场下离子交换色谱过程的吸附和洗脱过程及其传质特性 .结果表明 :在本文实验条件下 ,交变电场电流强度的变化对离子交换吸附平衡无显著影响 ,而外加电场在介质孔内所产生的电渗流可加速待分离组分进出固相介质的传递过程 ,显著提高色谱填充床的动态吸附容量 。

The chromatographic separation of BSA on DEAE-Sepharose Fast Flow in an alternating electric field is studied with emphasis on the adsorption and desorption and mass transfer characteristics. The experimental results show that, within the range of the electric field strength applied in the experiments described in this study, the application of the electric field has not shown a significant effect on ion exchange isotherm. However, the transport of the biological molecules among the liquid and solid phases can be greatly enhanced by electroosmosis generated inter-and intra-particle. This leads to an increased dynamic binding capacity and available bed volume for adsorption, consequently. The application of the alternating electric field also improves the elution peak properties described by the ratio of peak height to peak width and this is essential for obtaining a high resolution.