4-氨基吡啶分子模板聚合物选择性识别及结合性质的研究

Studies on the Binding Nature in Molecularly Imprinted Polymer Selective for 4-Aminopyridine

以 4 -氨基吡啶为模板分子制备了对 4 -氨基吡啶具有特异选择性的模板聚合物 .制得的棒状聚合物经研磨、过筛后 ,采用平衡结合方法评价了该模板聚合物的结合特性 .Scatchard分析表明 ,在所研究的浓度范围内 ,聚合物中形成了两类不同的结合位点 ,这两类结合位点的离解常数采用多点结合模型计算得到的值分别为 6.0 μmol/ L和 1 .2 mmol/ L.底物选择性表明 ,与其它结构相似的分子相比 ,该聚合物对模板分子4 -氨基吡啶显示了很强的结合能力 .1H NMR研究及 4 -氨基吡啶共振结构分析表明 。

A molecularly imprinted polymer was prepared using 4 aminopyridine as template. The bulk polymer obtained was ground up, sieved and investigated in equilibrium binding experiments to evaluate the binding characteristics of the 4 aminopyridine imprinted polymer. Scatchard analysis showed that two classes of binding sites were produced in the polymer matrix and their dissociation constants were calculated to be 6.0 μmol/L and 1.2 mmol/L respectively, by utilizing the model of multiple independent classes of binding sites. The substrate selectivity of the polymer was investigated. The results showed that the imprinted polymer exhibited much higher affinity for 4 aminopyridine among the tested compounds. \{ 1H NMR\} studies and the analysis of the resonance structure of 4 aminopyridine indicated that the selective binding interaction between the template and binding sites in the polymer arose from their ionic interaction.