毛细管电泳有效分离测定甜菊糖苷新方法的研究

Study of New Method for Separation and Determination of Stevia Glucosides by Capillary Electrophoresis

本文采用 1 0 0 %二甲基甲酰胺 ( DMF)作为样品的溶剂 ,以 Tris-硼砂作为分离甜菊糖苷的缓冲体系 ,探讨了进样量、柱温、电压、Tris-硼砂缓冲液浓度以及 p H值等条件对甜菊糖苷分离结果的影响。通过优化各种分离条件 。

This paper reported a new, effective and secure method for separation and determination of stevioside by capillary electrophoresis. The effects of different separation conditions on the migration time, selectivity and resolution were investigated. Under optimum separation conditions, the detector response for stevioside was linear over the range 0.05-9 mg/ml and the linear regression coefficient was 0.997. All components provided good resolution in 3.5 min. The relative standard deviations for the migration time of St and RA were 0.71% and 0.46%, respectively. This method was applied to the determination of steviol extract, and the content of St in this sample was 5.36%.