活化高分子膜免疫电极检测乳腺癌抗原

Determination of Cancer Antigen 15-3 Using an Activated Polymer Membrane Immunoelectrode

报道了一种灵敏度高和使用简便的免疫电极，用于测定乳腺癌抗原（ＣＡ１５－３）。ＣＡ１５－３抗体固定在用羟胺活化的聚氯乙烯（ＰＶＣ）－牛血清白蛋白（ＢＳＡ）基体膜上。当抗体膜与ＣＡ１５－３血清结合形成抗原体复合物膜后，将该复合物膜固定在自制 Ａｇ－ＡｇＣｌ基体电极顶端，利用毫伏计测定膜电位。该法可在１５～２４０Ｕ／ｍＬ范围测定ＣＡ１５－３，线性关系良好，回归方程：△ Ｅ－８．Ｍ＋１３．９７１ｇ［ＣＡ１５－３］，相关系数０．９９９８。人血清中其他常见抗原对ＣＡ１５．３测定基本无干扰。同时，探讨了免疫膜的电位响应机理。

A potentiometric immunoelectrode with non-labeled anticancer antigen CA 15-3 antibody used for determinating CA15-3 was reported. firstly, the anti-CA15-3 antibody was immobilized on the surface of polyvinyl chloride-bovine serum albumin membrane, which was activated previously. Secondly, CA15-3 was added on the surface of the antibody membrane to form the antigen-antibody complex. Lastly, the complex membrane was used to assemble an immunoelectrode with an Ag-AgCl inner electrode. The potential of the complex membrane was measured dth a pH meter. The response was linear over the range of 15 similar to 240 U/mL with a correlation coefficient of 0.9998. Other common antigens in human serum did not interfere with the determination of CA15-3. The mechanism of potential response was explored.