液相色谱/质谱/质谱联用法测定乌头属药材及中成药中乌头类生物硷含量

Determination of Aconitine alkaloids in Aconitum Plants and Related Proprietary Medicines by Liquid Chromatography/Mass Spectrometry/Mass Spectrometry

探讨了用ＬＣ／ＭＳ／ＭＳ联用法测定乌头属药材及有关中成药中乌头硷、次乌头硷和中乌头硷含量的方法。应用优化后的技术实验条件及采用多反应监测扫描方式，结果表明被分析样品中各乌头硷浓度在０．５～４０ ｍｇ／Ｌ范围内，色谱峰面积与浓度有良好的线性关系。此法亦应用于１４个不同的乌头属药材及１０个不同的中成药样品中的乌头硷含量检测。

A liquid chromatogtaphy/mass spectrometry/mass spectrometric method was developed for the determination of aconitine, mesaconitine and hypaconitine in root tuber of sevral species of Aconitum plants and related proprietary medicines. With the use of the optimized instrumental conditions and the Multiple Reaction Monitoring (MRM) technique, good linearity was obtained for the alkaloids in concentrations ranged from 0.5 to 40 mg/L. The method was applied to the analysis of 14 samples of various species of Aconitum plants and 10 different proprietary medicine samples.