高效液相色谱手性流动相添加剂分离乳酸对映体

High Performance Liquid Chromatographic Separation of Lactic acid Enantiomer Using Chiral Mobile Phase Additives

分别将β－环糊精、２，６－二甲基β－环糊精和２，３，６－三甲基β－环糊精作为手性流动相添加剂，系统地研究了Ｄ，Ｌ－乳酸在反相ＨＰＬＣ系统中的拆分，考察了流动相种类，ｐＨ值和手性流动相添加剂的浓度对手性分离的影响，建立了甲基化β－环糊精动态手性固定相法分离乳酸对映体的方法。

The enantiomeric resolution of lactic acid was studied by reversed high performance liquid chromatography. P-cyclodextrin (P-CD), dimethyl (DM) and TM-P-CD were used as chiral mobile phase additives. The effects of different mobile phase, pH and concentration of chiral mobile phase additives on resolution of D, L-lactic acid were investigated. The results showed: the enantioselectivity of TM-P-CD was better than those of P-CD and DM-P-CD. D, L-lactic acid could be partly separated by DM-P-CD and could not be separated by P-CD, while it could be fully separated by TM-P-CD. As the concentration of TM-P-CD changed from 0.20 mmol/L to 1.00 mmol/L, The resolution varied slightly. The experiment of the effect of pH of mobile phase on Rs showed: as pH changed from 2.4 to 3.0, the Rs remain stable, and when pH > 3.1, the Rs decreased with the increase of pH. The RSD of retention time of L-lactic acid and D-lactic acid were 0. 2% and 0. 2%, respectively. The chromatographic systems with a dynamically-generated stationary phase with 2, 3, 6-tri-O-methyl-beta -cyclodextrin proved to be an effective method for lactic acid enantiomer separation.