毛细管电泳──基因突变及多态性分析新方法

Capillary Electrophoresis——An Attractive Alternative To ol for Analyses of Genetic Mutations/Polymorphisms

着重介绍基因突变及多态性分析方法以及毛细管电泳在该领域中的应用。主要包括单链构象多态性分析，变性梯度及温度梯度电泳，杂合子分析，限制性片段多态性分析，等位基因特异性扩增，核酸杂交，引物扩展及小卫星和微卫星分析。

Recently, capillary electrophoresis (CE) has been successfully used for genetic analysis instead of conventional slab: gel electrophoresis. This article will give an overview of the fundamental aspects on mutation/polymorphism analyses in combination with capillary electrophoresis (CE), which mainly includes single strand conformation polymorphism analysis, denaturing gradient-gel electrophoresis, heteroduplex analysis, chemical mismatch cleavage, restriction fragment length polymorphism, allele specific oligonucleotide hybridization, allele specific amplification, primer extension, and minisatellite, and microsatellite analyses. A number of key applications are summarized.