高效液相色谱法测定血浆中酮洛芬的浓度

Determination on ketoprofen in plasma by HPLC

目的 :建立血浆中酮洛芬的HPLC测定法。方法 :色谱柱为SpherisorbC18柱 ,流动相为 5mmol·L-1磷酸盐缓冲液(pH 6 .8) 甲醇 (2 8∶72 ) ,流速 1ml·min-1,内标物为萘普生 ,在UV2 6 2nm波长处检测。结果 :在此分析条件下酮洛芬和内标分离完全且无其他干扰 ,保留时间分别为 12 .3和 9.5min。在 0 .0 1～ 5 .0 μg·ml-1浓度范围内酮洛芬峰面积比与其浓度间呈良好的线性关系 ,r=0 .9990 ,血浆中酮洛芬最低检测浓度为 3μg·ml-1,回收率 85 .9%～ 90 .9% ,日内、日间RSD分别为 1.4%～ 4.9%和 2 .5 %～ 6 .2 %。结论 :本法简便、灵敏 。

OBJECTIVE To establish an HPLC determination method for ketoprofen in plasma. METHODS Column: Spherisorb C 18 (100 mm× 4.6 mm, 3 μm), Mobile phase:5 mmol·L -1 of phosphate buffer(pH 6.8 ) methanol(28∶72, v/v), with flow rate of 1 ml·min -1 , and detection wavelength of 262 nm. The sensitivity was 0.005 auf, and the internal standard naproxen. 1 ml of plasma sample was taken for the preparation of testing sample.RESULTS Ketoprofen was completely separated from the internal standard, and no interference was found. The retention time was 12.3 and 9.5 min, respectively. The peak areas of ketoprofen within the concentration range between 0.01 ～ 5.0 μg·ml -1 showed good linearity to the concentrations, r= 0.9990 . The detection limit of keteprofen in plasma was 3 μg·ml -1 . The recovery was 85.9% ～ 90.9% , the variation within one day was 1.4% ～ 4.9% , and that between days was 2.5% ～ 6.2% . CONCLUSIONS This method is simple, sensitive.