喉癌微血管密度与细胞DNA含量参数的定量研究

Quantification of Microvascular Dentsity and Cell Nuclear DNA Content in Laryngeal Cancer

目的 :探讨喉癌微血管密度 (MVD)及细胞 DNA含量参数与患者预后的关系。方法 :采用F8特异性抗体和 SABC法测量 31例手术切除的喉癌组织的 MVD,用图像细胞分析术测量癌细胞的核面积峰值及 S期细胞数。结果 :MVD及 DNA含量在喉癌的组织学分级及分型中无显著性差异 (P>0 .0 5 ) ,MVD在 2年后的淋巴结转移组 (1 0 .2 3± 0 .78)与无淋巴结转移组 (6 .38± 0 .33)两者之间有显著性差异 (P<0 .0 5 ) ,核面积峰值和 S期细胞百分率在 2年后的淋巴结转移组 (6 1 .0 3± 0 .2 8) μm2 及 (38.1 1± 0 .37) %与无淋巴结转移组 (4 0 .97± 0 .41 ) μm2 及 (2 2 .97± 0 .2 9) %分别比较均有显著性差异 (P<0 .0 5 )。结论 :喉癌间质内的 MVD及喉癌细胞核的

Objective: To study the relationship between the microvascular density,cell nuclear DNA content and the prognosis in laryngeal cancer patient.Methods: In 31 cases,microvascular density was observed with anti factor Ⅷ antibody and SABC method,and the proliferating nuclear DNA content was examined with image cytometry.Results: The microvascular dencity and DNA content were not related to histologic grade and type of laryngeal cancer,but the differences of microvascular density were significant ( P <0.05) between the patients with metastasis (10.23±0.78) and these without metastasis (6.38±0.33) after two years.The measurement by the image cytometry showed that the metastasis groups had a mean nuclear peak area value of (61.03±0.28)μm 2 and the mean stage cell value of (38.11±0.37)%,and those of the groups without metastasis were (40.97±0.41)μm 2 and (22.97±0.29)%,respectively.There were significant differences ( P <0.05) between them.Conclusion: The angiogenesis and proliferating nuclear DNA content of laryngeal cancer are correlated with the lymph node metastasis and prognosis.