产溶葡球菌酶工程菌的发酵工艺

Fermentation Technology on Lysostapbin

对溶葡球菌酶生产菌ＢａｃｉｌｌｕｓＳｕｂｔｉｌｉｓ－００４１的发酵条件和工艺作了进一步研究和改进，该菌的摇瓶产酶可达５５０ｕ／ｍｌ，在５Ｌ自动发酵罐上达６８０ｕ／ｍｌ，且发酵周期大大缩短，仅５～６小时，发酵产率比原工艺提高一倍以上。滚工艺全部使用国产常用原料，为产业化生产提供便利。在研究中发现溶解氧对培养过程非常重要；ｐＨ对产酶没有明显影响；发酵中期流加葡萄糖可抑制蛋白酶对产物酶的降解作用。

n this experiment,the fermentation conditions have been widely investigated and improved inLysostaphin preducer B. subtilis 0044. In new condition,through 5-6hr.B. subtilis 0044 can produceLysostaphin 550u/ml in flask and 580u/ml in 5L fermenter.The Lysostaphin yield has increased by100% compared with original condition, The fermentation period has shortened.In the new technology,all components are from China it is suitable for industrial scale production.Through experirnent,itwas discovered that diss,olved oxygen is very improtant in Lysostaphin fermenta-tion,But pH is not adistinct effector.In the process of fermen tation, The addit ion of glucose or organic nitrogen can prevent decom positionof Lysostaphin by proteinase.