油甘超氧化物歧化酶纯化与性质

Purification and Some Properties of Superoxide Dismutase in phyl-lanthus emblica L.

本文以Ｔ氏液、磷酸钾和硫酸铰分级沉淀，ＳｅｐｈａｄｅｘＧ－１００柱分离方法，从油甘中提纯了超氧化物歧化酶。纯化倍数２９．４，比活性为２５０Ｕ／ｍｇ－ｐｒ。经聚丙烯酰胺凝胶电泳呈一蛋白带。该酶对Ｈ２Ｏ２敏感，而不受ＫＣＮ影响。经测定该酶含铁。用ＳＤＳ－ＰＡＧＥ测出亚基分子量为１８０００道尔顿。紫外吸收峰波长２７０ｎｍ。油甘ＳＯＤ的上述性质与Ｆｅ类ＳＯＤ相似。

n this paper iron-containing superoxide dismutase was purified from phyllanthus emblica L, by Tsuchi-hashi(T)reagent,potasslium phosphate,ammonium sulfate and sephadex G-100 column chromatogra-phy.Through the investigation of physiochemical properties, the purified SOD is homogeneous onPAGE. The specific activity is 250 u/mg pr and the stibtunit molecular weight is about 18 KD, A maxi-um absorption of the enzyme is found at 270 nm. The ptirified enzyme is sensitive to the inhibitionH2O2,on the contrary by KCN. This enzyme contains irons.These properties of the phyllanthus emblicaL SOD are very similar to those Fe-SOD.