摘要
本文以T氏液、磷酸钾和硫酸铰分级沉淀,SephadexG-100柱分离方法,从油甘中提纯了超氧化物歧化酶。纯化倍数29.4,比活性为250U/mg-pr。经聚丙烯酰胺凝胶电泳呈一蛋白带。该酶对H2O2敏感,而不受KCN影响。经测定该酶含铁。用SDS-PAGE测出亚基分子量为18000道尔顿。紫外吸收峰波长270nm。油甘SOD的上述性质与Fe类SOD相似。
n this
paper iron-containing superoxide dismutase was purified from phyllanthus emblica L, by
Tsuchi-hashi(T)reagent,potasslium phosphate,ammonium sulfate and sephadex G-100 column
chromatogra-phy.Through the investigation of physiochemical properties, the purified SOD is
homogeneous onPAGE. The specific activity is 250 u/mg pr and the stibtunit molecular weight
is about 18 KD, A maxi-um absorption of the enzyme is found at 270 nm. The ptirified enzyme
is sensitive to the inhibitionH2O2,on the contrary by KCN. This enzyme contains irons.These
properties of the phyllanthus emblicaL SOD are very similar to those Fe-SOD.
出处
《药物生物技术》
CAS
CSCD
1995年第1期28-32,共5页
Pharmaceutical Biotechnology
关键词
油甘
超氧化物歧化物
纯化
Phyllanthus emblica L
Superoxide dismutase
Purification