人血超氧化物歧化酶中试放大工艺探讨

Study on Middle Scale Preparation of Human Superoxide Dismutase

人血超氧化物歧化酶中试放大工艺探讨卢平，李清棠，刘鹏翰，杨珊（广西血液中心科研室，柳州５４５００５）关键词人血超氧化物歧化酶；制备超氧化物歧化酶（Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，简称ＳＯＤ）（ＥＣ．１．１５．１，１）是一种新型的抗炎酶制剂，它...

e reported a middle scale pre[paration of hSOD. After haemolysis of human red blood cell(RBC),the solution was precipitated by organ ic solven t to rid of haemogolbin and further purification by twotimes heat denaturation and UF. the specific activity of hSOD was more than 20000u/mg.It’s purifiea-tion was 191,6 times and the average recovery of activity was71.6%,20～50mg of hSOD was ob-tained from IL RBC using this method.