摘要
目的：探索胆囊收缩素（Ｃｈｏｌｅｃｙｓｔｏｋｉｎｉｎ，ＣＣＫ）、胃泌素（Ｇａｓｔｒｉｎ）对胆管癌细胞细胞内钙的调控作用。方法：应用细胞内钙测定技术，检测了ＣＣＫ、Ｇａｓｔｒｉｎ及其受体拮抗剂对胆管癌细胞株ＱＢＣ９３９细胞（简称ＱＢ细胞）细胞内钙的影响。结果：ＣＣＫ－８Ｓｕｌｆａｔｅｄ（ＣＣＫ－８Ｓ）、Ｇａｓｔｒｉｎ－１７（Ｇ－１７）明显促进了ＱＢ细胞内钙的释放；ＣＣＫ－Ａ受体拮抗剂Ｌ３６４，７１８（Ｌ１８）明显抑制了ＣＣＫ－８Ｓ对细胞内钙的激活，ＣＣＫ－Ｂ／Ｇａｓｔｒｉｎ受体拮抗剂Ｌ３６５，２６０（Ｌ６０）抑制了Ｇ－１７激发的细胞内钙释放。结论：细胞内钙参与了ＣＣＫ、Ｇａｓｔｒｉｎ对胆管癌细胞增殖影响的细胞内过程。
Objective: To explore the regulative effects of cholecystokinin (CCK) and gastrin on intracellular calcium in cholangiocarcinoma cells. Methods: The regulative effects of CCK8, gastrin17 and their receptor antagonists L18 and L60 on intracellular calcium in cholangiocarcinoma cell line QB. Results: CCK and gastrin stimulated the release of intracellular calcium in QB, but L18 and L60 inhibited the action of the two gut hormones. Conclusion: Intracellular calcium takes part in the process of intracellular action produced by CCK or gastrin.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1998年第1期30-31,共2页
Journal of Third Military Medical University
关键词
胆囊收缩素
胃泌素
细胞内钙
胆管癌
cholecystokinin
gastrin
intracellular calcium
cholangiocarcinoma cell
