缺氧时培养的心内膜内皮细胞活性肽分泌的改变

Effects of hypoxia on secretion of vascular activated peptide by cultured endocardial endothelial cells

目的：观察培养的新生小牛右心室心内膜内皮细胞（ＥＥＣ）血管活性物质的分泌功能及缺氧对其分泌的影响。方法：ＥＥＣ采用刮取法培养，用放免法测定培养液中血管活性肽的含量。结果：缺氧２４ｈ（ＰＯ２约为０．８ｋＰａ）使ＥＥＣ的内皮素（ＥＴ－１）和心钠素（ＡＮＰ）分泌显著增加（Ｐ＜０．０１），其分泌分别是常氧的１．９倍和１．６倍；缺氧也使ＥＥＣ分泌内源性洋地黄因子（ＥＤＬＦ）显著增加（Ｐ＜０．０５），其分泌是常氧的１．７倍，但缺氧使ＥＥＣ分泌血管紧张素（ＡＴⅡ）降低１８％（Ｐ＜０．０５）。结论：缺氧可引起ＥＥＣ多种血管活性因子的分泌发生改变。

Objective: To explore the effects of hypoxia on secretion of vascular activated peptide by cultured endocardial endothelial cells (EECs). Methods: After EECs were extracted from the right ventricule with scraping and cultured, the content of vascular activated peptides in the culture liquid was determined. Results: At the 24th h after hypoxia, the secretion of endothelin1 (ET1) and atrial natriuretic peptide (ANP) by EECs was significantly increased (P<0.01) to levels which were respectively 1.9 and 1.6 fold of those under normoxia. The secretion of endogenous digitalislike factor (EDLF) by EECs was also markedly increased (P<0.01) to a level which was 1.7 fold of that under normoxia but the secretion of angiotensin II (AT II) was decreased by 12%. Conclusion: Hypoxia results in changes of the secretion of various vascular activated factors by EECs.