培养大鼠皮层神经元创伤性损伤后NADPH-d组化反应研究

A study of NADPHd histochemistry after traumatic cortical neuronal injury in rats

目的：研究创伤性损伤后大脑皮层细胞ＮＡＤＰＨ－ｄ组化反应变化情况，探讨继发性脑损伤的某些机制。方法：用机械划痕法创伤性损伤混合培养１０ｄ的大鼠大脑皮层神经细胞，采用ＮＡＤＰＨ－ｄ组化染色和ＮＳＥ免疫组化染色法观察神经元和胶质细胞中的一氧化氮（ＮＯ）产生情况。结果：正常培养皮层神经元中有少量ＮＡＤＰＨ－ｄ阳性反应神经元，创伤性损伤后阳性反应神经元数量增加；胶质细胞中有ＮＡＤＰＨ－ｄ阳性反应，在创伤性损伤混合培养皮层神经元创道周围，ＮＡＤＰＨ－ｄ阳性反应明显强于其它部位。结论：创伤可致神经元和胶质细胞过量产生和释放ＮＯ并在继发性脑损伤中可能具有重要意义。

Objective: To study NADPHd histochemistry after traumatic cortical neuronal injury. Methods: After the mixcultured cortical neurons of SD rats were injured by mechanical trauma, the production of NO of cultured neurons was determined with NADPHd histochemistry and NSE immunohistochemistry. Results: A few cultured cortical neurons were NADPHdpositive neurons. After the injury, more cortical neurons became NADPHdpositive. NADPHdpositive reaction can also present in astrocytes and it is obviously stronger than anywhere else surrounding the traumatic tracts of cultured cortical neurons. Conclusion: The NO production can be induced by trauma and NO released from astrocytes and neurons might play an important role in secondary brain injury.