联合细胞毒诱导分化对胃癌SGC-7901细胞株作用的研究

Effects of differentiation inducers in combination with cytotoxic agents on human gastric carcinoma cell line SGC 7901

目的：探讨诱导分化剂联合细胞毒药物对胃癌细胞的作用效能，寻找治疗胃癌的新途径。方法：应用全反式维甲酸（ＡＴＲＡ）、α－干扰素（α－ＩＦＮ）和５－Ｆｕ，分别以单用、两两双用和联用对胃癌细胞系ＳＧＣ－７９０１细胞作用，用ＭＴＴ法、流式细胞术、图像分析和ＣＥＡ测定等方法观察细胞的形态和功能变化。结果：①随着单用、双用和联用对胃癌细胞的抑制率逐渐升高，平皿克隆形成率逐渐下降，细胞周期示Ｇ０／Ｇ１期细胞相对积聚，ＤＮＡ合成受抑制。②癌细胞恶性表型逐渐消失，成熟细胞特征逐渐增多，培养液ＣＥＡ滴度逐渐下降。③药物作用后细胞凋谢现象明显，可见大量凋谢小体。结论：３种药物联合使用时可对胃癌细胞产生显著的抑制增殖、诱导分化和促凋谢作用。

Objective: To investigate the effects of differentiation inducers in combination with cytotoxic agents on human gastric carcinoma cell line and explore a new approach of treatment to the cancer. Methods: The effects of ATRA in combination with α IFN and 5 Fu on human gastric carcinoma cell line SGC 7901 were observed in vitro. The gastric carcinoma cell line was exposed to the three agents in single, two agent combining or three agent combining manner respectively. The morphological and functional changes of the cell line were observed with MTT, flow cytometry, image analysis and CEA. Results: ①The cytostatic rates of the cell line were gradually increased while plating efficiency decreased after the treatment in three manners. The cells at G 0/G 1 phase were relatively increased and synthesis of DNA inhibited. ②The malignant phenotype of the cell line gradually disappeared while characteristics of mature cell were progressively increased. ③Obvious cell apoptosis and plenty of apoptotic bodies were seen after treatment. Conclusion: The combined use of the three agents can significantly inhibit proliferation, induce differentiation and promote apoptosis in the gastric carcinoma cells.