糖尿病患者高密度脂蛋白与受体结合的研究

The study of binding of high density lipoproteins from the diabetic patients to cell receptors using enzyme linked immunoreceptor assay

目的和方法：对糖尿病Ⅰ组（ＨｂＡ１ｃ为７０６％±０６０％）Ⅱ组（ＨｂＡ１ｃ为１１９７％±１６５％）各７例及对照组（ＨｂＡ１ｃ为４８５％±０２０％）６例的高密度脂蛋白（ｈｉｇｈｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎ，ＨＤＬ）糖化进行检测，同时将ＨＤＬ抗血清提纯为抗ＨＤＬＩｇＧ并用辣根过氧化物酶（ｈｏｒｓｅｒｄｉｓｈｐｅｒｉｏｘｉｄａｓｅ，ＨＲＰ）标记。用酶联免疫受体法测定ＨＤＬ与培养的人胚肺成纤维细胞（ｈｕｍａｎｌｕｎｇｆｉｂｒｏｂｌａｓｔ，ＨＬＦ）受体特异结合。结果：Ⅰ、Ⅱ组及对照组糖化值分别为３９２６±８１０、７２９４±６４０和２０４０±１１０Ｇｌｙｃｏｇｒｏｕｐ／ＨＤＬ，Ⅰ、Ⅱ组ＨＤＬ的细胞受体特异结合低于对照组（Ｐ＜００１），Ⅱ组低于Ⅰ组（Ｐ＜００１）。结论：糖尿病患者ＨＤＬ糖化高于正常人。

AIM and METHODS: The glycosylation of high density lipoproteins(HDL)from diabetic patient group I(HbA 1c =7.06%±0.60%, n =7).Ⅱ(HbA 1c =11.95%±1.65%, n =7)and control group(HbA 1c =4.85%±0.20%, n =6)was measured.Anti-HDL IgG was obtained from anti-serum to human HDL and labelled by horserdish perioxidase (HRP).The specific binding of HDL to human fibroblasts(HLF)receptors was measured using enzyme linked immunoreceptor assay. RESULTS: The glycosylated amount of HDL fromⅠ、Ⅱand control groups were 39.26±8.10,72.94±6.40,and 20.40±1.10 glycogroups/HDL,respecively.The specific binding of HDL to HLF receptors in group ⅠandⅡwas lower than that in control group,groupⅡwas lower than groupⅠ. CONCLUSLN: The glycosylation of HDL of diabetic patients is higher than that of normal subjects but lower in its specific binding to cultured cell receptors.