脂质过氧化在老化大鼠急性胆源性肝细胞线粒体损害中的作用及维生素E的影响

The role of lipid peroxidation in the aged hepatocyte mitochondria damage due to biliary sepsis and the protective effect of vitamin E

目的：探讨脂质过氧化在老化大鼠胆源性肝细胞线粒体受损中及维生素Ｅ（ＶＥ）的保护作用。结果：老化大鼠非ＶＥ处理组（ＮＶＥＧ）肝细胞线粒体丙二醛（ＭＤＡ）含量明显高于非老化组，超氧化物歧化酶（ＳＯＤ）和谷胱甘肽过氧化物酶（ＧＰＤ）活性明显低于非老化组，过氧化氢酶（ＣＡＴ）无明显变化。１８和２４月龄ＶＥ处理组（ＶＥＧ）ＭＤＡ显著低于ＮＶＥＧ，ＳＯＤ和ＧＰＤ显著高于ＮＶＥＧ，并以１８月龄组为明显。急性梗阻性左肝胆管炎（ＡＯＬＨ）后ＭＤＡ水平明显高于对照组，间接致伤肝叶（ＩＡＬ）较直接致伤肝叶（ＤＡＬ）为明显；ＩＡＬ线粒体ＳＯＤ和ＣＡＴ活性均显著高于对照组，ＤＡＬ显著降低；各叶ＧＰＤ活性均显著低于对照组，ＩＡＬ和ＤＡＬ均无显著差别；以上改变以２４月龄组为明显。各月龄ＶＥＧＡＯＬＨ２４ｈ时ＩＡＬＭＤＡ含量明显低于ＮＶＥＧ；１８月龄ＶＥＧＡＯＬＨ后ＳＯＤ水平均明显高于ＮＶＥＧ；各月龄ＶＥＧ组ＡＯＰＨ后ＧＰＤ和ＣＡＴ活性与ＮＶＥＧ比较均无明显差异。结论：脂质过氧化是老化大鼠胆源性肝细胞线粒体受损的重要机制，ＶＥ具有明显的保护作用。

AIM:The role of lipid peroxidation in the aged hepatocyte mitochondria damage deu to acute obstructive left hepatocholangitis (AOLH) and the protective effect of vitamin E(VE) were studied. RESULTS:The hepatocyte mitochondria malondialdehyde (MDA), catalase(CAT) and superoxide dismutase (SOD) were significantly increased following AOLH, especially in the indirect-attacked lobe (IAL). Glutathione peroxidase (GPD) activity was decreased in both IAL and direct-attacked lobe(DAL).The age groups had much more evident oxidative changes than the non-age group ( P <0.05) following AOLH. In VE pretreated groups,lower lipid peroxidation levels and better endogenous antioxidants reserves were found. CONCLUSIONS: An antioxidant/oxidant imbalance in hepatocyte mitochondria associated with AOLH and a vulnerability of mitochondria lipid peroxidation damage of the aged liver. Vitamin has protective effect on the age-related susceptibility of the hepatocyte michondria oxidant injury.