红细胞作载体的谷胱甘肽过氧化物酶用于脑缺血-再灌注的研究

Glutathione peroxidase encapsulated erythrocytes were used in the study of cerebral ischemia-reperfusion

目的和方法：采用红细胞（Ｅｒｙｔｈｒｏｃｙｔｅｓ，ＲＢＣ）作为谷胱甘肽过氧化物酶（ｇｌｕｔａｔｈｉｏｎｅｐｅｒｏｘｉｄａｓｅ，ＧＳＨ－Ｐｘ）的载体，将包载ＧＳＨ－Ｐｘ的ＲＢＣ（ＲＢＣ－ＧＳＨ－Ｐｘ）用于兔脑缺血－再灌注（ｉｓｃｈｅｍｉａ－ｒｅｐｅｒｆｕｓｉｏｎ，Ｉ－Ｒ）研究。结果：包入采用低渗透析法，渗透压达到１３２ｍＯｓｍ。采用５１Ｃｒ标记法测定的兔ＲＢＣ的循环半寿期为（１３４±１５）ｄ（ｎ＝６），ＲＢＣ－ＧＳＨ－Ｐｘ的半寿期为（７３±２５）ｄ（ｎ＝５）。２，３，５—氯化三苯基四氮唑（ＴＴＣ）染色结果表明，ＲＢＣ－ＧＳＨ－Ｐｘ对Ｉ－Ｒ损伤的脑组织有明显的保护作用。ＲＢＣ－ＧＳＨ－Ｐｘ对一氧化氮（ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）浓度的升高有抑制作用。

AIM and METHODS:The practicability using red blood cells(RBC)as the carrier of glutathione peroxidase(GSH-Px) was studied in this paper.GSH-Px was encapsulated into rabbit erythrocytes (RBC-GSH-Px),and was used in the study of cerebral ischemia-reperfusion(I-R) The effect of RBC-GSGH-Px on cerebral I-R was observed.RESULTS:The method of hypotonic dialysis was used in preparing RBC-GSH-Px,the osmotic pressure reached 132 mOsm.The circulation half-life (Τ1/2) of RBC-GSH-Px was determined in rabbits by 51 Cr radiolabel.Τ1/2=(13 4±1 5)d( n =6) for RBC,and Τ1/2=(7 3±2 5)d( n =5)d for RBC-GSH-Px.The increase of nitric oxide (NO) was inhibited by the administration of RBC-GSH-Px Staining with TTC showed that RBC-GSH-Px effectively protected cerebrum from I-R damage.CONCLUSION:Using RBC as the carrier of GSH-Px is practicable,it may be effective in the treatment of some brain diseases.