尿毒症患者血浆中分子物质对大鼠心肌舒缩性的抑制作用

Inhibitory effects of MMS from the plasma of uraemia patients on rat's myocardial contractility

目的：探讨尿毒症患者血浆中分子物质（ＭＭＳ），对大鼠心肌舒缩性的影响及心肌舒缩性改变的机制。方法：用凝胶层析法分离出ＭＭＳ，经尾静脉注入大鼠体内，实验用左心室插管术，采集信号经换能器数字化后输入微机，应用ＣＡＲＤＩＯ软件，分析注射ＭＭＳ前后心肌舒缩性的改变。结果：注射ＭＭＳ后，心室内压（ｐｅａｋ）下降，室内压达峰值时间（ｐｅａｋｔｉｍｅ）推迟，室内压最大上升变化率（ｄｐ／ｄｔｍａｘ）减小，室内压下降最大变化率（－ｄｐ／ｄｔｍａｘ）变慢，室内压下降最大变化速率所在时间（－ｄｐ／ｄｔｍａｘｔｉｍｅ）延长，上述变化在注射ＭＭＳ后１ｍｉｎ即出现，持续１２ｍｉｎ左右开始恢复。结论：ＭＭＳ可使心肌舒缩性能力减弱。

AIM:The effect of middle molecular substances (MMS) from the plasma of uraemia patients on rats' myocardial contractility and its mechanism was investigated.METHODS:MMS were separated from the plasma of uraemia patients by gel-chromatograpy intubation was performed on the left ventricle of rats. The intraventricular pressure signal was sampled via a transducer, digitized, and then transferred into a microcomputer to be analyzed with CADIO software before and after the injection of MMS into rats' tail veil.RESULTS :The intraventricular pressure peak was lowered, the peak time was delayed, both the maximum increasing rate (dp/dt max)and the decreasing rate (-dp/dt max)of intraventricular pressure were reduced, and the time -dp/dt max occurred was postponed. The indexes began to alter 1 min after the injection of MMS, and lasted 12 min or so, then restored. CONCLUSION:MMS could inhibit rats' myocardial contractility.