HBVS基因与IL-12基因联合免疫小鼠的免疫应答

Enhanced immune responses in BALB/c mice coimmunized with IL 12 gene expression plasmid and hepatitis B virus surface DNA based vaccine

目的 观察小鼠对 IL- 12基因表达质粒与 HBV S基因疫苗联合免疫的免疫应答 .方法 用已构建的 HBV S基因疫苗 (p CR3.1- S)和表达 IL- 12 p35和 p40蛋白的真核表达载体 p WRG316 9分别给 BAL B/c小鼠多点肌肉注射 ,2 wk后追加免疫 1次 ,用 EL ISA法及 MTT法检测小鼠血清抗 - HBs及脾细胞对 HBs Ag的特异性增殖反应 .结果 免疫接种 2 wk后小鼠血清抗 - HBs滴度及脾细胞对 HBs Ag的刺激指数均明显高于对照组 ,p WRG316 9+ p CR3.1- S组明显高于单纯p CR3.1- S注射组 .结论 IL- 12表达质粒可以增强 p CR3.1-S基因疫苗诱导的体液和细胞免疫应答强度 ;

AIM To study enhanced immune response in mice coimmunized with HBV surface DNA based vaccine and IL 12 gene expression plasmid. METHODS The HBV surface gene was cloned into a eukaryotic expression vector (pCR3.1 S). The Balb/c mice were immunized at multiple sites by intramuscular injection with pCR3.1 S and IL 12 gene expression plasmid (pWRG3169). Each mouse was boosted 2 weeks after the immunization. The anti HBs antibody was detected by ELISA and the stimulatory index of splenocytes on HBsAg was measured by MTT method. RESULTS The titer of anti HBs antibody and the stimulatory index of splenocytes from mice coimmunized with pWRG3169 and PCR3.1 S was significantly higher than that of the mice injected with pCR3.1 S alone. Antibody titer and SI of immunized mice were higher than those of the control group. CONCLUSION This study demonstrates that coimmunization of IL 12 gene expression plasmid and HBV surface gene can enhance humoral and cellular immune response of immunized mice.