用于分离EPA和DHA的新型HPLC固定相(Ⅱ)

Novel HPLC stationary phases for separation of EPA and DHA (Ⅱ)

为寻找从鱼油中分离高纯度二十碳五烯酸 (EPA)和二十二碳六烯酸 (DHA )的更有效方法 ,分别以 3,5 -二硝基苯甲酰氯和 2 -氯乙醇修饰的聚甲基丙烯酸甲酯(PMDVB)树脂微球 ,用作高效液相色谱 (HPL C)的固定相。均比修饰前的 PMDVB树脂微球分离效果好 ,比 Waters反相 C1 8键合硅胶柱分离因子大 ,柱容量高。以 3,5 -二硝基苯甲酰氯修饰的 PMDVB树脂效果最理想 ,以 2 -氯乙醇修饰的PMDVB树脂微球 ,造价低 ,工业上具有推广价值。作者对分离机理的研究表明 :缺电子基团 3,5 -二硝基苯甲酰氯与EPA、DHA形成电子转移络合物 ,电子转移络合物作用是一种弱的分子间作用力。

Porous PMDVB resin modified with electron deficient 3, 5 dinitro benzoylchloride and 2 chloride ethanol was prepared and applied as high performance liquid chromatography (HPLC) stationary phases for the separation of EPA and DHA from fish oil. Satisfied separation results were obtained. Its column loading capacity, separation factor and column life were better than those of waters reverse phase column. When porous PMDVB resin modified with 3, 5 dinitro benzoylchloride was applied as a stationary phase, the most desirable separation results were obtained. Porous PMDVB resin modified with 2 chloride ethanol is cheap. So it is very possible for large scale application to separate EPA and DHA in industry. Discuss the separation mechanism, we found that as electron lacked group, 3,5 dinitro benzoylchloride combinated to EPA and DHA with tran electron complex action, which is the weak action among molecular.